Miami Dade CSI Level 3 Detective Tim "Speed" Speedle (Rory Cochrane, seasons 1–2), trace evidence and impressions expert; originally from Syracuse, New York, with a degree in biology from Columbia University, Speed was killed in the line of duty in the third season premiere, Lost Son; he had not maintained his firearm diligently, and was shot by a suspect when it misfired during a police shoot-out. The character was written out at the request of Cochrane, who wanted to pursue a career in film and reportedly disliked the long, arduous shooting schedule for CSI: Miami.

Miami Dade Detective Yelina Salas (Sofia Milos, season 3, guest star seasons 1,2,5), a Colombian homicide detective frequently attached to CSI investigations, and the widow of Horatio's brother Raymond. She later starts a relationship with IAB Sergeant Rick Stetler, Horatio's personal nemesis. After her husband Raymond was revealed to still be alive, she left with him and their son for Brazil, where they planned to keep a low profile for Raymond's safety. She reappears in Season 5, starting with "Rio" (the season opener). Caine and Delko are in Rio de Janeiro to hunt down Antonio Riaz, who murdered Caine's wife (and Delko's sister) Marisol at the end of Season 4. Riaz is killed in a fight with Horatio, but not before killing Raymond Caine and enlisting Ray Jr into drug running in the shantytowns of the area. Yelina and Ray Jr wind up back in Miami, and Yelina goes into business as a private investigator (episode 522, "Burned").